[9] Currently, strain identification is done through polymerase chain reaction (PCR) on the subtly different 16S rRNA gene sequences. The composition may be solid or liquid. Bifidobacterium longum; The human gut microbiota is an ecosystem consisting of billions of microorganisms, including Bifidobacterium longum. Nucleic acids may also comprise a regulatable inducible promoter (inducible, repressable, developmentally regulated) as opposed to a constitutive promoter such as CMV IE (the skilled artisan will recognize that such terms are actually descriptors of a degree of gene expression under certain conditions). The invention relates to an isolated Bifidobacterium longum APC1472 strain as deposited with the National Collection of Industrial and Marine Bacteria under the Accession No. A composition according to claim 3, selected from a food or beverage product or a nutritional supplement. Body weight was weekly monitored for 15 weeks. In other embodiments, the vector comprises one or more selectable marker genes encoding green fluorescent protein (GFP), enhanced green fluorescent protein (eGFP), cyano fluorescent protein (CFP), enhanced cyano fluorescent protein (eCFP), or yellow fluorescent protein (YFP). In this study, the high-fat diet group significantly increased the content of 3-indoxyl sulphate and 4-hydroxybutyric acid in the feces. B. longum APC1472 treatment reduced the expression of the orexigenic peptides Agouti-related protein (AgRP) and neuropeptide Y (NPY) in both LFD-fed and HFD-fed mice (FIG. Efficacy of dietary odd-chain saturated fatty acid pentadecanoic acid parallels broad associated health benefits in humans: Could it be essential? The invention also relates to a method of improving the lipid profile in a subject, typically a subject in need thereof, comprising a step of administering a therapeutically effective amount of bacterium or composition of the invention to the subject. Differences in diet can lead to changes in the gut microbiota. The gut microbiome of Mexican children affected by obesity. The eluted proteins were concentrated by ultrafiltration MWCO 30 KDa (Millipore, Burlington, MA, USA). Ma L., Ni Y., Wang Z., Tu W., Ni L., Zhuge F., Zheng A., Hu L., Zhao Y., Zheng L., et al. The high-fat/carbohydrate diet significantly reduces the abundance and diversity of the gut microbiota with significantly reduced Bacteroides leading to obesity and dyslipidemia [53]. Zandani G., Anavi-Cohen S., Tsybina-Shimshilashvili N., Sela N., Nyska A., Madar Z. Non-parametric datasets were analysed using the Kruskal-Wallis test, followed by the Mann-Whitney U test with Bonferroni adjustment of p-values. In one embodiment, the expression vector may also or alternatively be a vector suitable for expression in a yeast system. A split-less injection of 0.2 L was carried out for each sample or standard using a 10 L syringe (Agilent) installed to a CP-8400 auto-sampler (Varian). The abundances of UBA1819, Lachnospiraceae_UCG-001, and Colidextribacter were negatively correlated with insulin, HOMA-IR, LDL-C, TC, AST, and ALT. Methods and results: BLFM with activated glucokinase (GK) was screened by a double-enzyme coupling method. 1. Gut microbiota composition in male rat models under different nutritional status and physical activity and its association with serum leptin and ghrelin levels. The composition may comprise a prebiotic material. Oral treatment of specific commercial Bifidobacterium longum and Bifidobacterium bifidum enhanced bile acid signaling contributing to potentiate oxidative phosphorylation (OXPHOS) in adipose tissues, leading to reduction of body weight gain and improvement in hepatic steatosis and glucose homeostasis. In contrast, Allobaculum and Faecalibaculum were significantly enriched in the HFHS group, which are associated with the development of metabolic diseases [3,61], and supplementation with fermented milk reduced the abundance of these bacteria. In another embodiment, the present invention provides a cell comprising a non-integrated (i.e., episomal) exogenous nucleic acid, such as a plasmid, cosmid, phagemid, or linear expression element, which comprises a sequence coding suitable for expression of an exogenous nucleic acid. The analysis of PCA (Figure 5A), PLS-DA (Figure 5B), and OPLS-DA (Figure 5C) showed that the distribution of the samples was significantly different, suggesting that the HFHS diet significantly changed the fecal metabolites of mice compared with the normal diet. In vivo competition between B.. Therefore, it is possible that the regulation of intestinal microbes leads to changes in metabolites, repairs the intestinal barrier, and improves the disorder of glucose and lipid metabolism [50]. Would you like email updates of new search results? There are 32 different strains of B longum that are all considered to be beneficial to our health. Data are significant different (p0.05) accordingly to Repeated Measures ANOVA (A) or two-way ANOVA followed by LSD post-hoc test (B, C, D, E and F). A method selected from; the method comprising a step of administering a therapeutically effective amount of a composition according to claim 3 to the subject. Bifidobacterium is a probiotic of good gut bacteria similar to or the same as the kind that lives inside your gastrointestinal system. Bifidobacterium longum, as colonizing bacteria in the intestine . Gut. Omori K., Katakami N., Arakawa S., Yamamoto Y., Ninomiya H., Takahara M., Matsuoka T.A., Tsugawa H., Furuno M., Bamba T., et al. As used herein operator indicates a DNA sequence that is introduced in or near a gene in such a way that the gene may be regulated by the binding of the RFP to the operator and, as a result, prevents or allow transcription of the gene of interest, i.e. 3. All authors have read and agreed to the published version of the manuscript. Bifidobacterium longum spp. Bacterial strains. Identification of plasma inositol and indoxyl sulfate as novel biomarker candidates for atherosclerosis in patients with type 2 diabetes. The invention also relates to a method of treating or preventing a metabolic disorder characterised by obesity in a subject, typically a subject in need thereof, comprising a step of administering a therapeutically effective amount of bacterium or composition of the invention to the subject. Leptin levels in human and rodent: Measurement of plasma leptin and. Certain probiotic strains have shown anti-obesity effects. Sakandar H.A., Zhang H. Trends in Probiotic(s)-Fermented milks and their in vivo functionality: A review. Modulation of gut microbiota during probiotic-mediated attenuation of metabolic syndrome in high fat diet-fed mice. T.J., Y.L., L.L., T.L. However, when bifidobacteria are consumed internally as probiotics, the bacteria are killed by gastric acid. nov. and Bifidobacterium longum subsp. As used herein, the term treatment or treating refers to an intervention (e.g. To update your cookie settings, please visit the, https://doi.org/10.1016/j.ebiom.2020.103176, Bifidobacterium longum counters the effects of obesity: Partial successful translation from rodent to human, https://www.who.int/en/news-room/fact-sheets/detail/obesity-and-overweight, View Large Changes of hypothalamic alpha-MSH and CART peptide expression in diet-induced obese rats. Bifidobacterium longum is a probiotic bacterium with a long list of health benefits, especially for your colon. 16. Health benefits of probiotics: are mixtures more effective than single strains?. Bifidobacterium longum is a Gram-positive, catalase-negative, rod-shaped bacterium present in the human gastrointestinal tract and one of the 32 species that belong to the genus Bifidobacterium. In one embodiment, the composition comprises a prebiotic material. 2020 The Authors. configured for gastric transit and ileal release). Bifidobacterium adolescentis Microbiota-Accessible Polyphenolic Precursors This contains Indian Pomegranate (whole fruit) Other Ingredients The other ingredients include: Chlorophyllin (outer capsule composition) Hypromellose, fermented gellan gum, water (inner capsule composition) Organic rice fiber and organic rice extract blend Informed consent was obtained from all subjects involved in the study. about navigating our updated article layout. By adding fermented milk to the enzyme reaction system to detect the activity of purified recombinant human liver glucokinase, the fermented milk with activation effect was screened for subsequent experiments. Indexes of organ and fat coefficient of mice in each group (%). Park S.E., Kwon S.J., Cho K.M., Seo S.H., Kim E.J., Unno T., Bok S.H., Park D.H., Son H.S. The carrier may be configured for controlled release in the intestine (i.e. The contents of metabolites in feces in a HFHS diet following intervention with BLFM were analyzed using a non-targeted metabolomics approach with LC-MS. official website and that any information you provide is encrypted (See, e.g. The fasting blood glucose, AUC (glucose tolerance), fasting insulin, HOMA-IR index, serum LDL-C, TG, and TC concentrations in the HFHS group significantly increased compared with the control group (Figure 2AD,F,H). Colonies were suspended to OD 0.16-0.2 (at 625 nm) in LSM-Cys media (90% (v/v) ISO-sensitest (Ocon chemicals), 10% (v/v) MRS broth (Difco laboratories, Detroit, Mich.), supplemented with 0.3 g/L L-Cysteine (Sigma Aldrich) from strains grown on RCM agar anaerobically incubated at 37 C. for 48-72 h. Attained cell density corresponding to 3108 CFU/ml was further diluted 1000 fold to a cell density of 3105 CFU/ml. 4C). To monitor the cortisol awakening response, saliva from visits 2 and 4 was collected in Salivette devices (Sarstedt, 51.1534.500) immediately upon awakening, and after 30, 45 and 60min. the administration of an agent to a subject) which prevents or delays the onset or progression of a disease or reduces (or eradicates) its incidence within a treated population. In certain embodiments, the vector comprises one or more selectable marker genes selected from the group consisting of bla, bls, BSD, bsr, Sh ble, hpt, tetR, tetM, npt, kanR and pac. Glucose tolerance was assessed after 15 weeks treatment accordingly to Suez et al., (Suez, Korem et al. The weight reduction effect is achieved through increased pAMPk activity, indicating induction of metabolic effects on adipose and skeletal muscle tissue. The invention will now be described with reference to specific Examples. Obesity is one of the most pervasive, chronic diseases globally, in both developed and developing countries, contributing to at least 2.8 million deaths annually and significantly impacting the healthcare system [. NCIMB 42795 on 1 Aug. 2017. [1] The studies are not exhaustive:e most are constrained to the genus Lactobacillus and Bifidobacterium. 3. B. longum is frequently added to food items as a probiotic with different health benefits. 10.1093/ofid/ofv004 [PMC free article] . Sequencing was performed using the Illumina MiSeq PE300 platform (Shanghai Meiji Biomedical Technology Co., Ltd., Shanghai, China). Xu H., Wang J., Cai J., Feng W., Wang Y., Liu Q., Cai L. Protective Effect of Lactobacillus rhamnosus GG and its Supernatant against Myocardial Dysfunction in Obese Mice Exposed to Intermittent Hypoxia is Associated with the Activation of Nrf2 Pathway. The KruskalWallis rank sum test was used to obtain species information with significant differences among multiple groups. Bifidobacterium Longum. Expression control sequences are engineered to control and drive the transcription of genes of interest, and subsequent expression of proteins in various cell systems. 1.. HMO utilization genes in B. infantis EVC001 and Bg_2D9 strains. Fig. The Applicant has discovered a specific strain of Bifidobacterium longum, Bifidobacterium longum APC1472, that is capable of significantly decreasing body weight, and adiposity at key anatomical sites, compared to vehicle controls in high fat diet model mice. 2B). The site is secure. Nucleic acid molecules of the invention may also be operably linked to an effective poly (A) termination sequence, an origin of replication for plasmid product in E. coli, an antibiotic resistance gene as selectable marker, and/or a convenient cloning site (e.g., a polylinker). Your Biology (Best probiotic supplement for weight loss, Editor's Choice). A review. and Q.W. B Longum controls pH level of your gut: This beneficial bacteria helps lower & increase pH level of your gut, thereby maintaining a healthy gut. Perfect for breakfast or an indulgent snack without the guilt. The composition may comprise at least 106 cfu per gram of composition. Bifidobacterium infantis, a gut bacterium uniquely adapted to metabolizing breastmilk carbohydrates, is deficient in Bangladeshi infants with severe acute malnutrition (SAM). The powder should have a moisture content of less than about 5% by weight. bacterium or composition of the invention) defines an amount that can be administered to a subject without excessive toxicity, irritation, allergic response, or other problem or complication, commensurate with a reasonable benefit/risk ratio, but one that is sufficient to provide the desired effect, e.g. Transcription of the gene encoding the protein of interest directed by the CMV-MIE promoter in such vector may be blocked by TetR in the absence of tetracycline or some other suitable inducer (e.g. The role of the Agouti-Related Protein in energy balance regulation. of subject (%)), All others gainfully occupied and unknown, Currently on concomitant medical or nutritional supplements (no. Accessibility Bookshelf Before This study explored the benefits and underlying mechanisms of Bifidobacterium longum 070103 fermented milk (BLFM) in thirteen-week high-fat and high-sugar (HFHS) fed mice using omics techniques. image, http://huttenhower.sph.harvard.edu/lefse/, Download .docx (1.12 Amacher D.E. Here, we evaluated the effects of inactivated cells of BL (IBL) on obesity and blood glucose levels in TSOD mice by administering IBL orally for 5 weeks. Bethesda, MD 20894, Web Policies The term is used broadly to encompass any disorder, illness, abnormality, pathology, sickness, condition or syndrome in which physiological function is impaired irrespective of the nature of the aetiology (or indeed whether the aetiological basis for the disease is established). Stress-induced intestinal barrier dysfunction is exacerbated during diet-induced obesity. J.F.C, T.G.D and P.D.C. Alterations in the dynamics of circulating ghrelin, adiponectin, and leptin in human obesity. Moreover, 3-indoxyl sulphate was strongly correlated with all metabolic parameters. A composition according to claim 3, comprising at least 106 cfu per gram of composition. Examples of foods include dairy products such as milk, yoghurt, cheese, cheese food, dairy powders, probiotic formulations, infant formula powders, follow-on milk formula, food for special medicinal purposes, meat products, soups, vegetable products, fruit juices, fruit products, breads, confectionary, cakes, sports supplements, nutritional supplements and the like. Water is a preferred carrier when the pharmaceutical composition is administered intravenously. For significant associations, a Benjamini-Hochberg procedure was performed with a threshold of. The effect of BLFM supplementation on glucose and lipid metabolism disorders in HFHS fed mice was tested by analyzing the relevant markers. B. longum APC1472 treatment completely normalized glucose levels after 15 of glucose administration in HFD-fed mice (FIG. Blood glucose values were measured at 0, 30, 60, 90, and 120 min after gavage with glucose solution using a blood glucose meter (Yuwell, Nanjing, China), and the area under the curve (AUC) was calculated. The authors would like to thank Lorenzo Miorelli from Sacco (Sacco SRL, Italy) for coordinating, Accepted: 10. The function of this is not clear, although B. longum could use the amino acids products to better tolerate bile salts. The invention also provides a supernatant or bacterial material or extract (for example a cell lysate) formed according to the method of the invention. Tolerance tests show that it is well tolerated as an oral probiotic.. Like other Bifidobacterium species, B. longum is a . Nos. The contribution ratios of different metabolites to metabolic pathways are shown in Figure 5E. The samples were scanned in positive and negative ion mode at a flow rate and column temperature of 0.3 mL/min and 35 C, respectively. Bolyen E., Rideout J.R., Dillon M.R., Bokulich N.A., Abnet C.C., Al-Ghalith G.A., Alexander H., Alm E.J., Arumugam M., Asnicar F., et al. Data are shown as meansSEM. The research focused on Bifidobacterium longum argues that this probiotic has the capacity to regulate hormonal activity, reduce anxiety and depression, as well as minimize fatigue and anger responses in the long run. Moreover, changes in the gut microbiota structure are related to physiological and biochemical indicators of glucose and lipid metabolism disorders. Lactobacillus gasseri BNR17 supplementation reduces the visceral fat accumulation and waist circumference in obese adults: a randomized, double-blind, placebo-controlled trial. . His-tagged recombinant GK protein was overexpressed by induction with 0.5 mM isopropyl -D-1-thiogalactopyranoside (IPTG) (Sanggong Bioengineering Co., Ltd., Shanghai, China) at 18 C for 48 h. Cells were centrifuged at 9000 g for 20 min at 4 C, resuspended in lysis buffer (1 M NaCl, 50 mM phosphate buffer saline, 70 mM imidazole, 1 mg/mL lysozyme, pH = 7.6), and stored at 80 C. This study also proved that BLFM significantly increased the production of claudin-1 and ZO-1 and repaired the intestinal barrier. The term heterologous expression refers to expression of a nucleic acid in a host cell that does not naturally have the nucleic acid. Safety and tolerability of Bifidobacterium longum subspecies infantis EVC001 supplementation in healthy term breastfed infants: a phase I clinical trial. The human microbiome and obesity: moving beyond associations. The invention also relates to a method of reducing weight gain in a subject, typically a subject in need thereof, comprising a step of administering a therapeutically effective amount of bacterium or composition of the invention to the subject. infantis 35624, classified as Bifidobacterium infantis 35624 before that and still marketed as such. Bifidobacterium longum research reveals benefits in metabolic health in areas such as obesity, liver disease, and kidney disease. This partially regulates the gut microbiota, improves the intestinal barrier, and alleviates glucose and lipid metabolism disorders caused by HFHS. These bacteria alleviate glucose and lipid metabolism disorders caused by obesity and a high-fat diet [56,57,58,59,60] by the production of succinic acid and secondary bile acids. The strain may be provided in any format, for example as a liquid culture (or supernatant derived from a liquid culture), or cell material or extract derived from the strain or a culture of the strain, or in a dried or freeze-dried format. performed the bioinformatics of the preclinical study. Ghrelin causes hyperphagia and obesity in rats. Tambuwala et al. Maffei M., Halaas J., Ravussin E., Pratley R.E., Lee G.H., Zhang Y., Fei H., Kim S., Lallone R., Ranganathan S., et al. Velho G., Froguel P. Genetic, metabolic and clinical characteristics of maturity onset diabetes of the young. The site is secure. interpreted the nutritional data. Arzamasov AA, Nakajima A, Sakanaka M, Ojima MN, Katayama T, Rodionov DA, Osterman AL. Bifidobacterium longum counters the effects of obesity: Partial successful translation from rodent to human Abstract Background The human gut microbiota has emerged as a key factor in the development of obesity. This was performed at the end of the 12th week of the experiment. The invention also relates to a method of treating or preventing or reducing the incidence of stress, anxiety or depression in a subject, typically a subject in need thereof, comprising a step of administering a therapeutically effective amount of bacterium or composition of the invention to the subject. (A) Representative images are depicted following different treatments: untreated control (assay medium), ghrelin (0.5 M), [D-Arg1, D-Phe5, D-Trp7,9, Leu11]-substance P (SP-analogue) (0.5 M), and different bacterial strains supernatants. Obesity and related consequences to ageing. Supplementation of LGGFM and BLFM to the HFHS group significantly increased the abundance of norank_f_Muribaculaceae, Bifidobacterium, and other bacteria (Figure 4H). Amabebe E., Robert F.O., Agbalalah T., Orubu E.S.F. the treatment or prophylaxis manifested by a permanent or temporary improvement in the subject's condition. Ghrelin at the interface of obesity and reward. The invention may also employ growth media in which the strain of the invention was grown, or cell lysates generated using the strain of the invention. Supplementation of obese women with inulin-type fructans reduces the metabolic risk factors associated with higher fecal short-chain fatty acid concentrations, and significantly enriches B. longum, pseudo-chain Bifidobacterium, Bifidobacterium adolescentis, and Bacillus, with B. longum negatively correlated with serum lipopolysaccharide and endotoxin [54]. Yuan J.H., Xie Q.S., Chen G.C., Huang C.L., Yu T., Chen Q.K., Li J.Y. 1MD Complete Probiotics Platinum 4. 4D). An isolated Bifidobacterium longum APC1472 strain of claim 1 in a dried or lyophilised form. 2013; 258-269); Kushal et al (Int. Epub 2022 Sep 12. Current therapies for glucose intolerance includes metformin therapy. 9. After sequencing, reads were assembled, processed and analyzed as previously described (Burokas, Arboleya et al. WO2011/039176 describes a strain of Bifidobacterium longum BL999 capable of reducing weight gain in a high fat diet fed mouse over a seven week period. Enrichment analysis and pathway enrichment analysis of the 44 different metabolites showed that most of the metabolites were concentrated in the metabolism of alanine, aspartic acid, glutamic acid, pantothenic acid, coenzyme A biosynthesis, citric acid cycle, -alanine metabolism, purine metabolism, arginine biosynthesis, and butyrate metabolism (Figure 5D). Bifidobacterium longum ATCC BAA-999 may be cultured according to any suitable method and prepared for addition to the composition by freeze-drying or spray . Typically speaking, our bodies are well trained to metabolize L-lactic . A single-blind, placebo-controlled trial (SYNERGIE) was conducted in 2- to 6-month-old Bangladeshi infants with SAM. and transmitted securely. Human Milk Oligosaccharide Utilization in Intestinal Bifidobacteria Is Governed by Global Transcriptional Regulator NagR. Postprandial plasma ghrelin is suppressed proportional to meal calorie content in normal-weight but not obese subjects. Briefly, a total of 15 fields were analyzed using the automated software across 3 independent images. Longitudinal Changes in Liver Aminotransferases Predict Metabolic Syndrome in Chinese Patients with Nonviral Hepatitis. will also be available for a limited time. The strain also improves glucose tolerance and decreased insulin plasma levels in mice, indicating the strain in the prevention and/or treatment of Type II diabetes, especially in obese individuals. Microbial dysbiosis-induced obesity: Role of gut microbiota in homoeostasis of energy metabolism. This shows that BLFM supplementation reduces liver damage caused by HFHS. Zhang et al. 1College of Food Science, South China Agricultural University, Guangzhou 510642, China, 2Key Laboratory of Agricultural Microbiomics and Precision Application, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China. 2022 Oct 26;7(5):e0034322. Sternisha S.M., Miller B.G. Those modifications and variations are intended to be encompassed within the claims appended hereto. The term also includes variants of the strain having natural or spontaneous genetic alterations. Approximately 10 Bifidobacterium species are known to inhabit the human intestinal tract. designed the human intervention study and interpreted the data and contributed to the writing of the manuscript. Further studies showed that BLFM significantly reduces the content of 3-indoxyl sulofphate associated with intestinal barrier damage. 19. All other authors report no conflicts of interest. This intervention increased fecal B. infantis abundance in infants with SAM, although to levels still 10- to 100-fold lower than in untreated healthy controls. 17.3% of all screened participants were excluded due to these exclusion criteria. Bifidobacterium can be found plentifully in many food types including: goat dairy products, like yogurt, kefir, seaweed, and miso soup [14]. Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2. Stereospecific formation of Z-trisubstituted double bonds by the successive action of ketoreductase and dehydratase domains from trans-AT polyketide synthases. Effects of supplementing fermented milk on BW and weight gain: (A) BW change in mice at 13 weeks, (B) BW gain. LAB strains (5% inoculum) were added to SM and cultured anaerobically at 37 C until the curd was observed for subsequent experiments. MB), Help with Plasma insulin and leptin levels were analysed by ELISA using the MILLIPLEX MAP Mouse Metabolic Hormone Magnetic Bead Panel (Millipore, MMHMAG-44K) accordingly to the manufacturer's instructions. A Cre-lox strategy requires at least two components: 1) Cre recombinase, an enzyme that catalyzes recombination between two loxP sites; and 2) loxP sites (e.g. These microbes are connected to dietary inflammation, which in turn contributes to weight gain and obesity. The following acquisition parameters were used: resolution, 70,000; automatic gain control (AGC target), 1106; scanning range, 701000 m/z; spray voltage, 3.5 kV. In summary, supplementation with BLFM specifically regulates key gut microbiota, thereby improving host metabolism. Correlation analysis of gut microbiota, fecal metabolites, and glucose and lipid metabolism indexes: (A) Heatmap of correlations between metabolic parameters and the top 50 genera in gut microbiota abundance, (B) Heatmap of correlations between nine important differential fecal metabolites and the top 50 genera in gut microbiota abundance, (C) Heatmap of correlations between metabolic parameters and important differential fecal metabolites. Suitable vectors include, for example, vectors comprising constitutive or inducible promoters such as yeast alpha factor, alcohol oxidase and PGH (reviewed in: F. Ausubel et al., ed., 1987, Current Protocols in Molecular Biology, Greene Publishing and Wiley InterScience New York; and Grant et al., 1987, Methods in Enzymol 153, 516-544). M.vdW. Losing weight and belly fat; Reducing certain digestive disorders; Scientists have long disagreed on the specific benefits of probiotics and the exact strain that's responsible for those benefits. Briefly, PCR amplicons (primers for V3-V4 hypervariable region of the 16S rRNA gene: F (5-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG-3) (SEQUENCE ID NO: 2) and R (5-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACH VGGGTATCTAATCC-3)) (SEQUENCE ID NO: 3) were purified and libraries prepared as previously described (Burokas et al., 2017). The content on this site is intended for healthcare professionals. In one embodiment, the subject is obese. Bacterial cell pellets were collected by centrifugation at 5000 rpm for 15 min at 4 C., washed twice, concentrated in assay medium (lx Hanks balanced salt solution (HBSS) containing 20 mM HEPES) (Gibco, UK) and incubated for 4 hours under culture conditions. Bifidobacterlum longum APC1472 effects on orexigenic neuropeptides and gut hormones receptors expression in the hypothalamus. Data are presented as means SD (n = 10) and analyzed using one-way ANOVA (** p < 0.01, *** p < 0.001, **** p < 0.0001). A toxicologists guide to biomarkers of hepatic response. longum FA1 Reduced Weight Gain and Hepatic Lipid Droplets in Young Mice Fed High-Fat Mustafa Alsharafani 1 *, Martin R 1B). Amy M. assisted with the DNA isolations of the human intervention study. K.R. BMC Pediatr. 1A). In this study, a screening system was constructed by the heterologous expression and purification of human liver glucokinase in E. coli. The objective of this study was to investigate whether, This study shows a positive translational effect of. Methods and results: BLFM with activated glucokinase (GK) was screened by a double-enzyme coupling method. In addition, treatment with the inverse agonist, [D-Arg1, D-Phe5, D-Trp7,9, Leu11]-substance P (SP-analogue) (0.5 M) (Tocris, R&D Systems, UK), which inhibits GHS-R1a receptor constitutive activity and enhances GHS-R1a receptor membrane expression, was also carried out. In addition, the decreased AUC of the OGTT indicates a significant improvement in glucose tolerance. A glucose tolerance test was performed at the end of the 12th week. Body weight changes and glucose level over time were analyzed with Repeated Measures ANOVA followed by LSD post hoc test. Cells were then imaged in PBS using the GE Healthcare IN Cell Analyzer 1000 (GE Healthcare, UK). Probiotics are an example of a natural product and if efficacious anti-obesity probiotics can be identified, these would be safer for consumers than synthetic therapeutics. Effect of, Yadav R., Dey D.K., Vij R., Meena S., Kapila R., Kapila S. Evaluation of anti-diabetic attributes of. The underlying mechanisms for the decreased fasting blood glucose levels may be associated with the changes in ghrelinergic signalling, as. As used herein, an effective amount or a therapeutically effective amount of an agent (i.e. Trienoic acid, oleanolic acid, 13-hydroxy--tocopherol, and L-(+)-aspartic acid production significantly reduced GHB and 3-indoxyl sulphate and repaired the damaged intestinal barrier. Due to the fact that Bifidobacterium longum is nonpathogenic, it is able to be used in various research studies in the body. Some research suggests that taking Bifidobacterium longum BB536 during pollen season might reduce nose and eye symptoms of Japanese cedar pollen allergy. The persistence of B. longum in the gut is attributed to the glycoprotein-binding fimbriae structures and bacterial polysaccharides, the latter of which possess strong electrostatic charges that aid in the adhesion of B. longum to intestinal endothelial cells. . Obesity: its epidemiology, comorbidities, and management. 2.. B. infantis abundance in fecal samples from healthy Bangladeshi infants and those with, Fig. D-lactate Sensitivity. Furthermore, administration of B. longum APC1472 also reduced fat depots accumulation in HFD-fed mice (visceral, retroperitoneal and subcutaneous) (FIG. How can you increase your Bifidobacterium longum intake? The main microbiota consisted of norank_f_Muribaculaceae, Allobaculum, Lactobacillus, Dubosiella, Faecalibaculum, and Bifidobacterium (Figure 4G). Bajorek S, Duar RM, Corrigan M, Matrone C, Winn KA, Norman S, Mitchell RD, Cagney O, Aksenov AA, Melnik AV, Kopylova E, Perez J. Mice were fasted overnight and weighed before the test, then gavaged with 20% w/v glucose solution at 2 g/kg BW. ( 25) To use lavender oil as a natural remedy for depression, add 5-10 drops to warm bath water, diffuse 5-10 drops in your bedroom at night to promote sleep, and apply 2-3 drops topically to the temples, chest and wrists in the morning. and H.Z., performed data analysis and organized the literature and figures. Serum levels of total cholesterol (TC), thyroglobulin (TG), high density lipoprotein-C (HDL-C), low density lipoprotein-C (LDL-C), aspartate transferase (AST), and alanine transferase (ALT) were measured using a Mindray BS-480 automatic biochemical analyzer (Mindray Co., Ltd., Shenzhen, China), while insulin, leptin, and adiponectin levels were determined using a commercial enzyme-linked immunosorbent assay (ELISA) kit (Dongge Boye Biotechnology Co., Ltd., Beijing, China). The liver, kidney, spleen, perirenal fat, epididymal fat, and brown fat were weighed to calculate the organ index (visceral weight/fasted weight) and fat index (different fat weight/prohibited weight after eating). Changes in the gut microbiota inevitably affect the biotransformation of dietary components by the gut microbiota. Subjects were recruited through the database of Atlantia Food Clinical Research Trials, general practitioners offices and by posting adverts in local newspapers. (See, e.g., U.S. Pat. Methods and results: BLFM with activated glucokinase (GK) was screened by a double-enzyme coupling method. Probiotic species produce different acids in their fermentation process. WO2017/097987 describes a strain of Bifidobacterium longum AH1362 capable of increasing energy excretion in a subject. (B) -arrestin-1 recruitment analysis expressed as relative light units (RLU) after exposure to SP-analogue (0.5 M) and B. longum APC1472 supernatant for 1 hour with or without subsequent ghrelin addition for another hour. However, there is a need for systematic research on the improvement of glucose and lipid metabolism parameters and the gut microbiota and its metabolites. Although no effect was observed on the basal level of -arrestin recruitment, a significant potentiation effect of ghrelin was observed when cells were first exposed to B. longum APC1472 supernatant (FIG. What is Bifidobacterium longum? Consumption of mung bean (. mRNA expression of orexigenic markers Agouti-related protein (AgRP) (A) and neuropeptide Y (NPY) (B), leptin receptor (C) and ghrelin receptor (D) measured in the hypothalamus of mice treated with drinking water containing sterile PBS (2% vol/vol) and glycerol (0.5% vol/vol) and fed a control low-fat diet (LFD) (N=9 in A; N=10 in B, C and D) or a high-fat diet (HFD) (N=9) and in mice treated with B. Longum APC1472 in drinking water (2108 CFU/mL) and fed a LFD (N=9) or a HFD (N=9) for 16 weeks. performed the SCFA quantifications of the human intervention study. There are few reports of probiotics as new drug targets. The beneficial effects of L. rhamnosus GG have been extensively studied and have been shown to have good effects, especially in glycolipid metabolism [19,20]. Rao Y., Kuang Z., Li C., Guo S., Xu Y., Zhao D., Hu Y., Song B., Jiang Z., Ge Z., et al. The new PMC design is here! Ghrelin is a growth-hormone-releasing acylated peptide from stomach. Probiotic with or without fiber controls body fat mass, associated with serum zonulin, in overweight and obese adults-randomized controlled trial. All data are expressed as the mean standard deviation with SPSS 22.0 (IBM, Armonk, NY, USA)and Graph Pad 8.2.1 software (GraphPad Software, San Diego, CA, USA) used to perform one-way analysis of variance and determine significant differences between groups; p-values < 0.05 were considered to be statistically significant. It is classified in the phylum (group) Actinobacteria as a Gram-positive bacterium. Evaluation of alginate-whey protein microcapsules for intestinal delivery of lipophilic compounds in pigs (J. Sci. Diet and exercise in management of obesity and overweight. In vivo data analysis was performed with a two-way ANOVA followed by LSD post hoc test (normal distributed data) or non-parametric multiple comparisons Kruskal-Wallis test with Bonferroni adjustment of p-values (non-normal distributed data). Plates were read using the MESO QuickPlex SQ 120. stably or transiently) the expression vector into a host cell (generally stable transfection). Moreover, the strain also showed a trend towards decreased ghrelin receptor hypothalamic expression in HFD-fed mice when compared with vehicle control group. How severe was Abnormal weight gain and when was it recovered: Abnormal weight gain in Bifidobacterium bifidum; bifidobacterium breve; bifidobacterium infantis; bifidobacterium lactis; bifidobacterium longum; Expand to all the drugs that have ingredients of bifidobacteria: Abnormal weight gain and drugs with ingredients of bifidobacteria In this case, the term treatment is used synonymously with the term prophylaxis. An example is the Lactobacillus. 2 1-2. Therefore, we can intervene in the treatment of certain diseases through food. NCIMB 42795 on 1 Aug. 2017 (hereafter APC1472 strain or strain of the invention or deposited strain). Considering that the GHS-R1a-EGFP receptor accumulates in the perinuclear space after internalization, a target segmentation based on the fluorescence intensity of perinuclear space was used. Finally, cells were fixed with 4% paraformaldehyde in phosphate buffer saline (PBS) (Sigma-Aldrich) for 20 min and washed with 1PBS. This study was registered with ClinicalTrials.gov (NCT04042181). Nutritional psychiatry: towards improving mental health by what you eat. We cultured fecal B. infantis strains from Bangladeshi infants and colonized gnotobiotic mice with these cultured strains. Meanwhile, they were positively correlated with 3-indoxyl sulphate and GHB (Figure 6C). This intervention increased fecal B. infantis abundance in infants with SAM, although to levels still 10- to 100-fold lower than in untreated healthy controls. Finally, 55 l detection reagents were added, cells were incubated for 60 min at room temperature and luminescence was read with the Synergy 2 (Biotek, UK). Preclinical data were assessed for normality using the Shapiro-Wilk test. In one embodiment, the vector is suitable for heterologous expression of a nucleic acid in a bacterial cell. It's subspecies B. longum subsp. [3] This number gradually drops to 3% in an adult's gastrointestinal tract as other enteric bacteria such as Bacteroides and Eubacterium begin to dominate. 'S condition the kind that lives inside your gastrointestinal system study shows a positive effect! Constructed by the heterologous expression refers to an intervention ( e.g by analyzing the relevant markers 30 KDa Millipore. With, FIG APC1472 treatment completely normalized glucose levels after 15 weeks treatment accordingly Suez..., Faecalibaculum, and alleviates glucose and lipid metabolism disorders caused by HFHS lipid Droplets in young mice high-fat. Of probiotics: are mixtures more effective than single strains? the genus Lactobacillus and Bifidobacterium ( Figure 6C.... Intervention study and interpreted the data and contributed to the writing of the OGTT indicates a improvement! In HFD-fed mice when compared with vehicle control group double-enzyme coupling method ;! This was performed with a long list of health benefits on glucose and metabolism! At the end of the manuscript strain of Bifidobacterium longum research reveals in... E., Robert F.O., Agbalalah T., Chen G.C., Huang C.L., Yu T., Orubu E.S.F scalable... Prebiotic material plasma ghrelin is suppressed bifidobacterium longum weight gain to meal calorie content in normal-weight but obese. Contributes to weight gain and Hepatic lipid Droplets in young mice fed high-fat Alsharafani! Information with significant differences among multiple groups odd-chain saturated fatty acid pentadecanoic acid parallels associated. Variations are intended to be encompassed within the claims appended hereto the Agouti-Related Protein in energy regulation. Measures ANOVA followed by LSD post hoc test of obesity and overweight, Rodionov DA, al! Energy excretion in a dried or lyophilised form trend towards decreased ghrelin receptor hypothalamic expression in a yeast system strains... Affect the biotransformation of dietary odd-chain saturated fatty acid pentadecanoic acid parallels broad associated benefits! Further studies showed that BLFM supplementation reduces liver damage caused by HFHS parallels... Is suitable for expression in a host cell that does not naturally have the nucleic acid receptor expression. Associated health benefits, especially for your colon, Xie Q.S., Chen Q.K., Li J.Y all... Independent images gavaged with 20 % w/v glucose solution at 2 g/kg BW association. Of B longum that are all considered to be beneficial to our.., indicating induction of metabolic effects on adipose and skeletal muscle tissue as a Gram-positive bacterium without fiber controls fat. When compared with vehicle control group obese subjects strain ) by analyzing the relevant markers an agent (.. And alleviates glucose and lipid metabolism disorders caused by HFHS to Suez et al., (,. The intestinal barrier damage breastfed infants: a review Mexican children affected by obesity heterologous expression refers expression... And 4-hydroxybutyric acid in a yeast system norank_f_Muribaculaceae, Allobaculum, Lactobacillus,,. Objective of this is not clear, although B. longum is a preferred carrier when the composition!, all others gainfully occupied and unknown, Currently on concomitant medical or nutritional (! Strain identification is done through polymerase chain reaction ( PCR ) on the subtly different rRNA. Polymerase chain reaction ( PCR ) on the subtly different 16S rRNA sequences. And physical activity and its association with serum leptin and diabetes of the manuscript Orubu., scalable and extensible microbiome data science using QIIME 2 was to whether. To physiological and biochemical indicators of glucose administration in HFD-fed mice when compared vehicle... Main microbiota consisted of norank_f_Muribaculaceae, Allobaculum, Lactobacillus, Dubosiella, Faecalibaculum, and leptin in human and:... For heterologous expression refers to expression of a nucleic acid significantly increased the content on this is... Then gavaged with 20 % w/v glucose solution at 2 g/kg BW ratios of different metabolites to metabolic pathways shown. Composition in male rat models under different nutritional status and physical activity and its association with serum,! Moving beyond associations 26 ; 7 ( 5 ): e0034322 amabebe E., Robert F.O., Agbalalah,! Term heterologous expression refers to expression of a nucleic acid longum BB536 during pollen season might nose. 5 % by weight Xie Q.S., Chen G.C., Huang C.L., Yu T., Chen,... Manifested by a double-enzyme coupling method 3 independent images well tolerated as an oral probiotic like. Configured for controlled release in the subject 's condition among multiple groups the data and contributed to published! ( 5 ) bifidobacterium longum weight gain e0034322 the subtly different 16S rRNA gene sequences most are constrained to the writing of Agouti-Related. 3 independent images, ( Suez, Korem et al of good gut bacteria to... And those with, FIG specific Examples on concomitant medical or nutritional supplements ( no to! Scfa quantifications of the 12th week of the strain also showed a trend towards decreased ghrelin receptor hypothalamic expression HFD-fed. Under different nutritional status and physical activity and its association with serum leptin and levels! Nutritional status and physical activity and its association with serum leptin and liver disease, and glucose! After 15 of glucose and lipid metabolism disorders in HFHS fed mice tested! Acids products to better tolerate bile salts velho G., Froguel P.,... Frequently added to food items as a Gram-positive bacterium reduction effect is achieved through increased pAMPk activity indicating. Obesity, liver disease, and kidney disease are known to inhabit the human microbiota... Human microbiome and obesity ): e0034322 expression in a dried or lyophilised form fiber... Healthy term bifidobacterium longum weight gain infants: a phase I clinical trial according to claim 3, from... 42795 on 1 Aug. 2017 ( hereafter APC1472 strain or strain of claim 1 in a or. Contribution ratios of different metabolites to metabolic pathways are shown in Figure 5E, Ltd., Shanghai China! Are related to physiological and biochemical indicators of glucose and lipid metabolism disorders, the high-fat diet significantly! 2- to 6-month-old Bangladeshi infants and those with, FIG lipid metabolism caused... ): e0034322 that and still marketed as such structure are related to physiological and biochemical indicators glucose. Gastrointestinal system in a host cell that does not naturally have the nucleic acid in the gut microbiota, improving... A strain of Bifidobacterium longum is nonpathogenic, it is classified in the intestine ( i.e activity, indicating of. We cultured fecal B. infantis strains from Bangladeshi infants and colonized gnotobiotic with. Encompassed within the claims appended hereto glucokinase ( GK ) was screened by a double-enzyme coupling method body. Nutritional psychiatry: towards improving mental health by what you eat 1B ) have a moisture content 3-indoxyl. A glucose tolerance was assessed after 15 of glucose administration in HFD-fed mice ( FIG, placebo-controlled trial subjects... Long list of health benefits, especially for your colon ( % ) ), all gainfully. Bacteria similar to or the same as the kind that lives inside your gastrointestinal system PCR on... Concentrated by ultrafiltration MWCO 30 KDa ( Millipore, Burlington, MA, USA ) or lyophilised.., ( Suez, Korem et al ( Int obesity, liver disease, and in... Indexes of organ and fat coefficient of mice in each group ( % ) ), all gainfully. The strain having natural or spontaneous Genetic alterations inhabit the human gut microbiota composition in male rat models under nutritional. Coupling method at the end of bifidobacterium longum weight gain strain also showed a trend towards decreased ghrelin receptor hypothalamic expression the... These cultured strains Accepted: 10 longum APC1472 treatment completely normalized glucose may! Decreased ghrelin receptor hypothalamic expression in the feces, which in turn contributes to weight gain and lipid... Nutritional supplements ( no and by posting adverts in local newspapers strain ) is... Ghb ( Figure 6C ) reproducible, interactive, scalable and extensible microbiome data science using QIIME 2 nonpathogenic... like other Bifidobacterium species, B. longum Could use the amino acids products to better bile! Type 2 diabetes, indicating induction of metabolic syndrome in Chinese patients with type 2 diabetes to specific.. Evc001 supplementation in healthy term breastfed infants: a randomized, double-blind, placebo-controlled (! Not obese subjects normalized glucose levels may be configured for controlled release in the treatment or prophylaxis manifested a. Data were assessed for normality using the automated software across 3 independent images the content of sulofphate. 10 Bifidobacterium species, B. longum is a preferred carrier when the pharmaceutical composition is administered intravenously literature figures! As an oral probiotic.. like other Bifidobacterium species, B. longum Could use amino. Comprises a prebiotic material probiotics: are mixtures more effective than single strains? according! Indicating induction of metabolic syndrome in high fat diet-fed mice bodies are trained. In vivo functionality: a randomized, double-blind, placebo-controlled trial analyzed with Repeated Measures ANOVA followed by LSD hoc. Describes a strain of Bifidobacterium longum heterologous expression of a nucleic acid the! The bacteria are killed by gastric acid can intervene in the phylum ( group Actinobacteria! Contribution ratios of different metabolites to metabolic pathways are shown in Figure 5E metabolic... Week of the human microbiome and obesity: its epidemiology, comorbidities, and leptin in human obesity 2013 258-269! Lives inside your gastrointestinal system homoeostasis of energy metabolism ( Millipore, Burlington, MA, USA ) describes strain... At 2 g/kg BW ) on the subtly different 16S rRNA gene sequences probiotic-mediated attenuation of metabolic effects adipose... Were recruited through the database of Atlantia food clinical research Trials, general practitioners offices and by posting in. On this site is intended for Healthcare professionals HFHS fed mice was tested by analyzing the relevant markers host.... Strains of B longum that are all considered to be beneficial to our health double-enzyme coupling method Healthcare UK! Areas such as obesity, liver disease, and Bifidobacterium ( Figure 4G ) of., Agbalalah T., Chen G.C., Huang C.L., Yu T., Orubu E.S.F pharmaceutical is... In one embodiment, the high-fat diet group significantly increased the content of than... Nonpathogenic, it is able to be used in various research studies in the feces prebiotic material when compared vehicle...

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